The ultrafiltration devices uses an ultrafiltration membrane made of polyethersulfone (PES), which has the function of rapid concentration and buffer exchange. The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for Multi-fold concentrated. Typical processing time is 15 to 60 minutes depending on Molecular Weight Cutoff (MWCO)Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. The concentrate is collected from the filter device sample reservoir using a pipetter, and the device can be spun in a swinging bucket or fixed angle rotor. The devices are supplied non-sterile and are for single use only.