Reactive Species: Human
Endotoxin: <2EU/mL
Appearance: Brown liquid
ActCel T Cell Activation Reagent is synthesized by coupling a nanomatrix with anti-human CD3 antibody and anti-human CD28 antibody, and it can gently and efficiently activate T cells or PBMCs (Peripheral Blood Mononuclear Cells).
Experimental Procedures:
1.1 Conduct the experiment at a titer of 1:200. The specific addition amounts are shown in the following table:
| Culture Plate |
Maximum Culture Volume |
Total T Cell Number |
Volume of T Cell Activation Reagent Added per Well |
| 96-well |
0.2 mL |
0.3×10⁶ |
1 μL |
| 48-well |
1 mL |
1×10⁶ |
5 μL |
| 24-well |
2 mL |
2×10⁶ |
10 μL |
| 12-well |
4 mL |
4×10⁶ |
20 μL |
| 6-well |
5 mL |
5×10⁶ |
25 μL |
1.2 Taking 1×10⁶ T cells as an example: Add 995 μL of X-VIVOTM 15 medium and 5 μL of T cell activation reagent (containing 200 IU/mL IL-2) to 1×10⁶ T cells. Then culture them at 37 °C under 5% CO₂ for 2 days.
1.3 After culturing for 2 - 3 days, centrifuge at 300g for 10 minutes, remove the supernatant, and add 2 mL of fresh complete medium to remove the T cell activation reagent. Then continue culturing at 37 °C under 5% CO₂.
1.4 Every 2 days, pipette the cell suspension thoroughly to mix evenly, add 2 times the volume of fresh complete medium (the ratio of the original volume to the supplementary volume is 1:2), and count the cells to calculate the amplification factor. Then culture them in a 37 °C, 5% CO₂ incubator. For example, if the medium is supplemented on day 4, and the original volume is 2 mL, 4 mL of medium should be added, and the final total volume will be 6 mL.
- When incubating the T cell activation reagent with cells, it is necessary to mix them thoroughly to improve the activation efficiency.
- The titer of the T cell activation reagent can be adjusted within the range of 1:100 - 300.
- The time for centrifuging to remove the T cell activation reagent can be optimized according to customer requirements.
This product is only applicable to in vitro cell culture and cannot be directly used for clinical treatment.