Specifications
Expression Host: E.coli
Synonyms: FGF2, FGFB, FGF basic, HBGF-2
Molecular Weight: Predicted 17.1kD
Purity: >90% (SDS-PAGE)
Endotoxin: <0.1EU/μg
Purification: Chromatography
Form: White lyophilized powder
Bioactivity
Validated by HUVEC proliferation assay:
Specific Activity ≥1×10⁶ IU/mg
Intended Use
The Heat Stable Recombinant Human bFGF Protein is an engineered variant of native bFGF. It retains the critical biological functions of:
• Supporting pluripotency in human embryonic stem cells
• Stimulating proliferation of mesoderm/ectoderm/endoderm-derived cells
• Promoting cell migration & angiogenesis
Key innovation: Enhanced thermal stability allows:
✓ Structural integrity across broad temperature ranges
✓ Sustained bioactivity under routine culture conditions
Reconstitution Protocol
- Dissolve in sterile water/saline/culture medium/PBS
- Aliquot at ≥100μg/mL concentration
Storage:
• -20°C: 6 months
• -80°C: 12 months
Avoid repeated freeze-thaw cycles
Important Notes
▲ For research use only. Not for clinical administration.
Storage Conditions
• Long-term: -20°C (24 months stability)